In vitro studies of calcium phosphate glass ceramics with different solubility with the use of human bone marrow cells.

Two glass ceramics in the CaO--P2O5--MgO system with the incorporation of K2O or TiO2 oxides were prepared with the goal of using them as potential bone graft substitutes. The incorporation of TiO2 and K2O led to the preparation of specific crystalline phases in the structure of the glass ceramics, which show different degrees of biodegradation. In fact, the 45CaO--45P2O5--5MgO--5K2O has been previously demonstrated to be much more soluble in aqueous solutions than the 45CaO--37P2O5--5MgO--13TiO2 glass ceramic. The in vitro biological activity of the two calcium phosphate glass ceramics was studied with the use of human bone marrow osteoblast cell cultures maintained for 28 days, and seeded materials were assessed for cell proliferation and function. The Ti-containing glass ceramic showed a stable surface throughout the culture time, on macroscopic and SEM observation. Osteoblast cells proliferated gradually, especially during the third week, with a high alkaline phosphatase activity and formation of a mineralized matrix. On SEM observation, attached cells appeared with a spread-polygonal morphology typical of the osteoblast cells, with extensive cell-to-cell contact. Cell behavior on the seeded material was similar to that found on cultures performed on tissue-culture-grade polystyrene; except for the presence of lower cell numbers during the first 2 weeks. By contrast, the K-containing glass ceramic showed a highly instable surface with dissolution/precipitation processes occurring throughout the culture time. Few cells adhered to the material surface, and subsequent proliferation was also hindered, especially from the first week onwards. Cell numbers were significantly lower than those observed in the Ti-containing glass ceramic during most of the incubation time. Results suggest that the different in vitro biological behavior of these two glass ceramics is mainly due to the significant differences in the surface degradation rate, which is directly correlated to the chemical composition of the mother glass.